Table 5.
Identification of Borrelia proteins by Ge-LC-MS/MS in mouse skin infected with Borrelia afzelii isolate NE4049.
| Mouse ID # | 1 | 2 | 3 | 4 |
|---|---|---|---|---|
| Spirochete density fla/104 gapdh | 876 | 602 | 1231 | 723 |
| Mouse proteins | 4489 | 4156 | 3961 | 4372 |
| Flagellin | X (11) | X (8) | X (8) | X (10) |
| Flagellar filament outer layer | X (1) | X (4) | X (1) | X (6) |
| VlsE | X (3) | X (2) | X (2) | X (2) |
| L-lactate dehydrogenase | X (3) | X (5) | X (4) | |
| GAPDH | X (4) | X (5) | X (7) | X (6) |
| Enolase | X (3) | X (1) | X (2) | |
| Oligopeptide ABC transporter gi|111115153 | X (3) | X (2) | X (1) | X (6) |
| Neutrophil activating protein | X (3) | X (1) | ||
| 2,3-biphosphoglycerate-dependent phosphoglycerate mutase | X (2) | X (1) | X (2) | X (3) |
| Elongation factor Tu | X (3) | X (7) | X (2) | X (9) |
Mice were infected via tick bite (10 nymphs) and the Borrelia infection was reactivated with clobetasol at 40 days post-infection. Proteins were identified using both Mascot and OMSSA algorithms. The numbers of identified peptides are in brackets. Of the 36 proteins identified, only those identified with at least 3 different peptides in at least one biopsy are listed.