Table 6.
Targeted detection of bacterial proteins by LC-SRM in mouse skin infected with Borrelia burgdorferi ss N40 or Borrelia afzelii NE4049.
| Detected proteins | B. burgdorferi ss N40 | B. afzelii NE4049 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Infection Mode | IP | ID | ID | ID | ID | ID | ID | ID | |
| Spirochete density fla/ 10 4 gapdh | 259 | 43 | 15 | 10 | 304 | 69 | 10 | 6 | |
| Sequence of SRM-monitored peptides | Mouse 1 | Mouse 2 | Mouse 3 | Mouse 4 | Mouse 5 | Mouse 6 | Mouse 7 | Mouse 8 | |
| DbpA | DITDEIDAIK | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. |
| GVNFDAFK | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| TTANGIIEIVK | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| TVTDAAEQHPTTTAEGILEIAK | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| VSENSFILEAK | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| Flagellin | ANLGAFQNR | Q (45) | Q (5) | N.d. | N.d. | Q (36) | Q (4) | N.d. | N.d. |
| AINFIQTTEGNLNEVEK | Q (94) | N.d. | N.d. | N.d. | Q (52) | N.d. | N.d. | N.d. | |
| INTPASLSGSQASWTLR | Q (88) | Q (14) | N.d. | N.d. | Q (77) | N.d. | N.d. | N.d. | |
| ASDDAAGMGVSGK | D | D | N.d. | N.d. | D | N.d. | N.d. | N.d. | |
| ELAVQSGNGTYSDSDR | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| GSIQIEIEQLTDEINR | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| IADQAQYNQMHMLSNK | D | D | N.d. | N.d. | D | N.d. | N.d. | N.d. | |
| MIINHNTSAINASR | D | D | N.d. | N.d. | D | N.d. | N.d. | N.d. | |
| NNAINAANLSK | N.d. | N.d. | N.d. | N.d. | D | D | N.d. | N.d. | |
| NNGINAANLSK | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| NSTEYAIENLK | N.d. | N.d. | N.d. | N.d. | D | N.d. | N.d. | N.d. | |
| TAEELGMQPAK | D | D | N.d. | N.d. | D | N.d. | N.d. | N.d. | |
| HP BAPKO_0593 | LPLALNLAVSR | N.d. | N.d. | N.d. | N.d. | D | N.d. | N.d. | N.d. |
| Lipoprotein gi|365823350 | EFFDWLSK | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. |
| GEALSLFFQK | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| SLTEIDSGNGIPLVVSDVVK | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| VLTESESNNELK | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| Vls13 | AAAAVSSVSGEQILK | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. |
| AVSSVSGEQILK | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| GIVDAAGTAAGK | D | D | D | N.d. | D | N.d. | N.d. | N.d. | |
| GIVDAAGTAAGKK | D | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| IGESADNGAAADADSVK | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| VAAALVLR | D | D | N.d. | N.d. | D | N.d. | N.d. | N.d. | |
| Vls2 | AAEAVSSVSGEQILK | D | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. |
| AAEEAIVGATGDGTK | D | D | N.d. | N.d. | N.d. | N.d. | N.d. | N.d. | |
| Borrelia species typing | YES | YES | YES | NO | YES | YES | NO | NO | |
Growth of spirochetes was reactivated with clobetasol at 40 days after intraperitonal (IP) or intradermal (ID) inoculation. N.d. = Not detected, D = Detected, Q = Detected and quantified. Quantifications are in brackets (fmol/mg skin).