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. 2017 Dec 1;7:16766. doi: 10.1038/s41598-017-16919-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental Design. All ecotypes (anadromous (Little Campbell River), marine (Oyster Lagoon), freshwater (Trout Lake)) were hatched and raised in salinities of 0 ppt and 20 ppt for 5 months at 12 L:12D photoperiod. After 5 months, starting on 11/23/14, the light period was decreased at a rate of 20 minutes per week and reached a minimum of 8 L:16D (solid grey shading) at 2/24/15 for all groups. Over the same period, the temperature in the experimental tanks was decreased by 0.13 °C per day, reaching a minimum of 4 °C on 3/4/15, while the control tanks were maintained at 17 °C.