Table 2.
Bacterial counts following peptoid/peptide treatment for 1 hour and before SEM and TEM sample preparation and DNA retardation assay results. Partially-killed samples are shown in bold.
| Compound | SEM | TEM | Retardation assay (µg of compound)* | |
|---|---|---|---|---|
| Bacterial count after 10 µM treatment (% of control) | Bacterial count after 10 µM treatment (% of control) | Bacterial count after 100 µM treatment (% of control) | ||
| [control] | 5 × 105 (100) | 1 × 108 (100) | 1 × 108 (100) | ND |
| pexiganan | 2 × 10 1 (0.004) | 8 × 10 4 (0.08) | 0 (0) | 0.6 |
| melittin | 2 × 10 5 (40) | 1 × 108 (100) | 3 × 10 7 (30) | 1.8 |
| fowlicidin-1 | ND | 4 × 10 5 (0.4) | ND† | ND† |
| LL-37 | ND | 5 × 10 7 (50) | 4 × 10 6 (4) | 2.0 |
| 1 | 2 × 10 5 (40) | 2 × 10 5 (0.2) | 0 (0) | 3.0 |
| 1 achiral | ND | 1 × 108 (100) | 3 × 10 4 (0.03) | 1.5 |
| 1-NLys5,11 | ND | 1 × 108 (100) | 3 × 10 7 (30) | 1.0 |
| 1-Pro6 | 5 × 105 (100) | 1 × 108 (100) | 3 × 10 6 (3) | 7.0 |
| 1-Nsna6,12 | ND | 2 × 10 5 (0.2) | 0 (0) | 2.0 |
| 1 17mer | 3 × 10 1 (0.006) | 3 × 10 5 (0.3) | 0 (0) | 9.0 |
| 2 | 5 × 105 (100) | 1 × 108 (100) | 1 × 10 8 (100) | >145§ |
*The mass of compound required to completely retard migration of 1 µg of DNA. †We did not have enough fowlicidin-1 to evaluate these conditions. §DNA was partially, but not completely, retarded at a peptoid/DNA weight ratio of 145. Note: ND signifies not determined.