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. 2017 Dec 1;7:16779. doi: 10.1038/s41598-017-16872-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RGC numbers in mid-peripheral and peripheral regions of retinas derived from SD/SD and SA/SA mutant mice. (a) Brn3a-positive RGCs were counted in mid-peripheral and peripheral regions of retinas obtained from young (■) and ageing (□) SD/SD (left) and SA/SA (right) mutant mice. Paired factorial ANOVA and Bonferroni post hoc test, **P < 0.01. (b) Ratios of RGC number in the retina at the periphery to that at the mid-periphery. The ratios (MP ratio) were calculated for WT and homozygous young and ageing SD/SD (left) and SA/SA (right) mutant mice. Factorial ANOVA and Bonferroni post hoc test, *P < 0.05. (a,b) Mid-peri, mid-peripheral region; Peri, peripheral region; Young, young mice; Old, ageing mice. Sample numbers are shown in parentheses. Data are means ± s.d.