Table 2.

Characteristics of primers designed for analyzing genetic diversity of Zanthoxylum cultivars.

Code	Primer pair	Length (bp)	Melting temperature (°C)	Product size (bp)	GC content (%)
ZB-2	F: AACAAATGGTGCTGATGACA	20	54	177	45.2
	R: ATTACTGCTCCTCGAAACTGG	21
ZB-4	F: TCAACAGATATGGCTCGAGT	20	54	206	43.2
	R: TTTGAAACCCCGAACATGG	19
ZB-11	F: AGGCTTTCAATGAGTTCAAGG	21	56	206	43.7
	R: CTCTTCTGGATTTTCCTCGT	20
ZB-28	F: ACAACAGACTGCTAAGCCAA	20	54	139	39.6
	R: TCCTTTTGCCTTTCAACCC	19
ZB-30	F: ACACTGATATACCGGCACCT	20	58	201	49.8
	R: ATGCCTCGTTTGGATCGCTT	20
ZB-32	F: ATTCCGAAATCCCGCCCAA	19	56	180	47.2
	R: TTTCCGACAGCAACGGCTT	19
ZB-43	F: CAGTCTCATTAAACGCTCC	19	54	214	40.2
	R: TCTTCTCTTCATCCGCTTC	19
ZB-69	F: CCTACGCCAACTCACC	16	58	149	65.1
	R: ATGCGAACCTTCAACGTC	18
ZB-73	F: TTGAGGACGACGAAGATG	18	56	201	51.4
	R: CTTCTTCCCCAGTCTCTC	18
ZB-89	F: GATGTTGCGAATGATACAAG	20	54	100	46
	R: GACAATATTCAGGAGCAGGA	20
ZB-97	F: AGTATTGTGGAGCTAACAT	19	54	191	38.2
	R: TGAGGCCATCTTTACACT	18