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. 2017 Dec 1;7:16745. doi: 10.1038/s41598-017-16841-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activity characterization of the improved variants and bioremediation potential. (A) Phosphotriesterase catalytic efficiency comparison between wt-SsoPox and the best selected variants. Data for wt-SsoPox are from Hiblot et al.⁴⁶. (B) Determination of the reaction time for hydrolyzing 95% using the αsD6 variant of a solution of pesticide at 250 µM. Two enzyme-to-substrate ratios ( $[E]/ [S]$ ), 10⁻² and 10⁻³, were considered.