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. 2017 Dec 1;7:16745. doi: 10.1038/s41598-017-16841-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Loop 8 conformations in improved mutants. Active site loop 8 region of mutant structures superimposed with the wt-SsoPox structure. The active site location is indicated by the presence of the bi-metallic catalytic center shown as spheres. The wt-SsoPox structure loop 8 conformation is shown as dark green cartoon. (A) αsA1′s loop 8 is shown in orange cartoon, (B) αsD6′s loop 8 is shown in green cartoon, and is not entirely visible in the electronic density maps, (C) αsC6′s loop 8 is shown in blue cartoon, (D) αsB5′s loop 8 is shown in yellow cartoon, and is not entirely visible in the electronic density maps, (E) αsA6-CC’s loop 8 is shown in magenta cartoon, (F) αsA6-OC’s loop 8 is shown in cyan cartoon.