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. 2017 Dec 1;7:16745. doi: 10.1038/s41598-017-16841-0

Table 2.

Kinetic characterization of SsoPox variants.

Substrate Sso Pox k cat (s −1 ) K M (µM) K I (µM) k cat /K M (M −1 .s −1 ) Enhancement/wt
Ethyl-paraoxon (I) wt° 1.26 ± 0.13 × 101 2.43 ± 0.37 × 104 — 5.12 ± 1.31 × 102 1
αsA1 2.70 ± 0.23 × 101 8.00 ± 1.55 × 102 — 3.37 ± 0.71 × 104 65
αsA6 5.87 ± 0.47 1.63 ± 0.63 × 103 — 3.61 ± 1.43 × 103 7
αsB5 2.06 ± 0.14 × 101 6.01 ± 0.96 × 102 — 3.43 ± 1.46 × 104 67
αsC6 4.27 ± 0.25 × 101 1.50 ± 0.16 × 103 — 2.86 ± 0.35 × 104 55
αsD6 3.22 ± 0.18 × 101 1.09 ± 0.12 × 103 — 2.95 ± 1.55 × 104 58
αsB5 W263M * * — 5.62 ± 0.11 × 103 11
αsB5 W263L * * — 8.07 ± 0.28 × 103 16
αsB5 W263I 4.09 ± 0.89 × 101 6.64 ± 1.75 × 103 — 6.16 ± 5.07 × 103 12
W263L+ * * — 2.37 ± 0.33 × 103 5
W263M+ 6.82 ± 0.57 9.31 ± 1.63 × 102 — 7.33 ± 1.42 × 103 14
Ethyl-parathion (II) wt° ND ND ND ND ND
αsA1 ND ND ND ND ND
αsA6 3.20 ± 0.20 × 10−2 1.34 ± 0.18 × 102 3 250 ± 1020 2.39 ± 0.35 × 102 ND
αsB5 2.69 ± 0.15 × 10−1 2.72 ± 0.49 × 102 — 9.86 ± 3.14 × 102 ND
αsC6 8.53 ± 0.30 × 10−3 7.70 ± 1.10 × 101 — 1.10 ± 0.16 × 102 ND
αsD6 7.35 ± 0.36 × 10−1 5.34 ± 0.62 × 102 — 1.38 ± 0.57 × 103 ND
αsB5 W263M 5.24 ± 0.23 × 10−2 2.02 ± 0.31 × 102 — 2.59 ± 0.74 × 102 ND
αsB5 W263L 4.35 ± 0.16 × 10−2 3.46 ± 0.37 × 102 — 1.26 ± 0.44 × 102 ND
αsB5 W263I 4.74 ± 0.21 × 10−2 2.61 ± 0.37 × 102 — 1.82 ± 0.57 × 102 ND
W263L ND ND — ND ND
W263M ND ND — ND ND
Methyl-paraoxon (III) wt° 2.71 ± 0.64 2.14 ± 0.68 × 103 — 1.27 ± 0.70 × 103 1
αsA1 4.35 ± 0.87 × 101 1.90 ± 0.52 × 103 — 2.29 ± 0.78 × 104 18
αsA6 5.89 ± 0.55 5.46 ± 1.02 × 102 — 1.08 ± 0.23 × 104 8
αsB5 * * — 4.31 ± 0.14 × 103 3
αsC6 2.36 ± 0.37 × 101 7.59 ± 2.04 × 102 — 3.11 ± 0.97 × 104 25
αsD6 4.25 ± 0.52 × 101 2.08 ± 0.34 × 103 — 2.04 ± 0.42 × 104 16
Methyl-parathion (IV) wt° 1.10 ± 0.02 × 10−3 1.21 ± 0.10 × 102 — 9.09 ± 0.90 1
αsA1 1.30 ± 0.50 × 10−2 3.53 ± 0.34 × 102 — 3.68 ± 1.46 × 101 4
αsA6 1.55 ± 0.15 × 10−2 2.54 ± 0.39 × 102 1 520 ± 384 6.10 ± 1.11 × 101 7
αsB5 1.50 ± 0.04 × 10−1 1.58 ± 0.15 × 102 — 9.49 ± 0.94 × 102 104
αsC6 3.00 ± 0.10 × 10−3 1.21 ± 0.15 × 102 — 2.48 ± 0.32 × 101 3
αsD6 6.89 ± 0.35 3.43 ± 0.44 × 102 — 2.01 ± 0.28 × 104 2210
Malathion (V) wt° 8.90 ± 0.40 × 10−4 1.60 ± 0.29 × 102 — 5.56 ± 1.26 1
αsA1 7.10 ± 0.40 × 10−4 2.22 ± 0.35 × 102 — 3.20 ± 0.54 0.5
αsA6 ND ND ND ND ND
αsB5 1.84 ± 0.16 × 10−2 9.13 ± 1.61 × 102 — 2.02 ± 1.01 × 101 4
αsC6 2.47 ± 0.44 × 10−2 3.20 ± 1.90 × 101 820 ± 343 7.72 ± 4.78 × 102 139
αsD6 1.00 ± 0.02 × 10−1 1.13 ± 0.10 × 102 — 9.08 ± 2.00 × 102 163
αsB5 W263M 8.92 ± 0.67 × 10−3 8.61 ± 1.32 × 102 — 1.04 ± 0.51 × 101 2
αsB5 W263L 1.11 ± 0.08 × 10−2 9.28 ± 1.32 × 102 — 1.20 ± 0.60 × 101 2
αsB5 W263I 1.18 ± 0.08 × 10−2 7.80 ± 1.08 × 102 — 1.51 ± 0.71 × 101 3
W263L ND ND — ND ND
W263M ND ND — ND ND
Chlorpyrifos (VI) wt ND ND — ND ND
αsD6 * * — 3.12 ± 0.09 × 102 ND
αsB5 * * — 2.07 ± 0.05 × 102 ND
αsB5 W263M * * — 1.64 ± 0.03 × 102 ND
αsB5 W263L * * — 1.46 ± 0.03 × 102 ND
αsB5 W263I * * — 1.42 ± 0.03 × 102 ND
W263L ND ND — ND ND
W263M ND ND — ND ND
Diazinon (VII) wt ND ND — ND ND
αsD6 8.93 ± 0.79 2.30 ± 0.31 × 103 — 3.89 ± 2.56 × 103 ND
αsB5 1.26 ± 0.06 4.10 ± 0.65 × 102 — 3.03 ± 0.98 × 103 ND
αsB5 W263M 3.89 ± 0.38 × 10−1 3.91 ± 1.04 × 102 — 9.92 ± 3.65 × 102 ND
αsB5 W263L 5.61 ± 0.23 × 10−1 4.74 ± 0.53 × 102 — 1.18 ± 0.44 × 103 ND
αsB5 W263I 4.01 ± 0.30 × 10−1 3.24 ± 0.72 × 102 — 1.24 ± 0.42 × 103 ND
W263L ND ND — ND ND
W263M ND ND — ND ND
Fenitrothion (VIII) wt ND ND — ND ND
αsD6 7.94 ± 0.50 × 10−1 5.28 ± 0.80 × 102 — 1.50 ± 0.62 × 103 ND
αsB5 1.40 ± 0.20 × 10−2 3.14 ± 1.43 × 102 — 9.11 ± 1.40 × 101 ND
αsB5 W263M 2.22 ± 0.12 × 10−2 3.31 ± 0.53 × 102 — 6.69 ± 2.31 × 101 ND
αsB5 W263L 2.63 ± 0.13 × 10−2 6.54 ± 0.75 × 102 — 4.01 ± 1.79 × 101 ND
αsB5 W263I 2.01 ± 0.08 × 10−2 3.97 ± 0.46 × 102 — 5.05 ± 1.81 × 101 ND
W263L 3.66 ± 0.15 × 10−3 4.15 ± 0.44 × 102 — 8.82 ± 3.32 ND
W263M 9.49 ± 0.43 × 10−4 1.57 ± 0.28 × 102 — 6.06 ± 1.55 ND
Fensulfothion (IX) wt ND ND — ND ND
αsB5 4.28 ± 0.39 × 10−2 9.41 ± 1.72 × 102 — 4.55 ± 2.30 × 101 ND
αsD6 4.06 ± 1.60 × 10−1 8.60 ± 3.97 × 103 — 4.72 ± 4.04 × 101 ND
αsB5 W263M * * — 1.12 ± 0.46 × 101 ND
αsB5 W263L 4.94 ± 1.35 × 10−2 2.57 ± 1.04 × 103 — 1.92 ± 1.30 × 101 ND
αsB5 W263I * * — 6.50 ± 0.07 ND
W263L ND ND — ND ND
W263M ND ND — ND ND
Coumaphos (X) wt ND ND — ND ND
αsD6 * * — 1.64 ± 0.02 × 104 ND
αsB5 * * — 7.82 ± 0.14 × 103 ND
αsB5 W263M * * — 1.15 ± 0.02 × 103 ND
αsB5 W263L * * — 1.16 ± 0.05 × 103 ND
αsB5 W263I * * — 1.11 ± 0.04 × 103 ND
W263L * * — 6.01 ± 0.18 ND
W263M * * — 4.80 ± 0.32 ND
3-oxo-C10-AHL ( l ) (XI) wt° * * — 3.16 × 104 1
αsA1 * * — 1.41 × 102 0.004
αsA6 * * — 1.78 × 103 0.06
αsB5 ND ND — ND ND
αsC6 * * — 5.84 × 102 0.02
αsD6 ND ND — ND ND
Undecanoic-γ-lactone (XII) wt° * * — 2.36 × 103 1
αsA1 * * — 1.63 × 103 0.7
αsA6 * * — 4.40 × 105 186
αsB5 * * — 9.05 × 102 0.4
αsC6 * * — 5.73 × 105 243
αsD6 * * — 1.60 × 104 7
Undecanoic-δ-lactone (XIII) wt° * * — 7.86 × 104 1
αsA1 * * — 2.55 × 103 0.03
αsA6 * * — 9.65 × 106 123
αsB5 * * — 5.33 × 103 0.08
αsC6 * * — 3.60 × 105 5
αsD6 * * — 7.50 × 104 0.9

*Corresponds to undetermined values because enzyme saturation could not be reached.

ND corresponds to undetectable values because substrates were not hydrolyzed or too poorly hydrolyzed to be observable.

°Data taken from Hiblot et al.46.

+Data taken from Hiblot et al.11.