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. 2017 Dec 1;7:16747. doi: 10.1038/s41598-017-17140-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qRT-PCR validation of 15 selected DEGs involved in carbohydrate metabolism in response to CGMMV infection. Informed by the RNA-Seq analysis, 15 DEGs were selected for validation via qRT-PCR analysis. The watermelon 18 S rRNA gene was used as internal control. Error bars represent mean ± standard deviation (SD) and the data are averages of three biological replicates. Asterisks indicate statistically significant differences compared to control (Student’s t-test): *P < 0.05, **P < 0.01.