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. 2016 Jun 21;34(6):713–723. doi: 10.1007/s10719-016-9706-y

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Binding of anti-SSEA-1 monoclonal antibody MC480 (a) and anti-Le^x monoclonal antibody P12 (b) to non-acid glycosphingolipids of undifferentiated hESC. Lane 1, hESC SA181 non-acid glycosphingolipid fraction IV; Lane 2, reference total non-acid glycosphingolipids of human blood group AB erythrocytes; lane 3, reference Le^x pentaosylceramide. (c) Flow cytometry diagram of hiPSC incubated with antibodies against CD15 (C3D-1; grey), SSEA-1 (MC480; red), and Le^x (P12; blue)