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. 2016 Jun 17;34(6):797–806. doi: 10.1007/s10719-016-9699-6

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Comparison of HPLC profiles of glycans derived from adipose-derived hMSCs lot#: 2118 P3 and P28. a Anion-exchange HPLC profiles. S0, S1, S2, and S3 are standard N-glycans containing 0, 1, 2, and 3 Sia residues. b Size-fractionation HPLC profiles of neutral fraction (N), monosialylated N-glycan fraction (A1), and disialylated N-glycan fraction (A4). Following anion-exchange HPLC, further purification was performed. Each peak was pooled, and when necessary, was further purified by reversed-phase HPLC, or subjected to further structural analysis. Arrowheads indicate the peak numbers