Table 1.

The three most commonly used screening methodologies for mutation screening of KRAS, NRAS, BRAF and PIK3CA in the last three EQA years

Scheme year/gene	Most common methodology	Second most common methodology	Third most common methodology
2013–2014
KRAS	Roche cobas®	Pyrosequencing	Sanger sequencing
NRAS	Pyrosequencing	Sanger sequencing	Mass spectrometry
BRAF	Roche cobas®	Pyrosequencing	Sanger Sequencing
PIK3CA	Roche cobas®	Mass spectrometry (Sequenom)	Pyrosequencing
2014–2015
KRAS	Sanger sequencing	Roche cobas®	Pyrosequencing
NRAS	Sanger sequencing	Pyrosequencing	NGS
BRAF	Sanger sequencing	Pyrosequencing	NGS
PIK3CA	NGS	Sanger sequencing	a
2015–2016
KRAS	Sanger sequencing	NGS	Pyrosequencing
NRAS	Pyrosequencing	Sanger sequencing	NGS
BRAF	NGS	Pyrosequencing	Sanger sequencing
PIK3CA	NGS	Sanger sequencing	Mass spectrometry (Sequenom)

^aPyrosequencing, Roche cobas® and mass spectrometry (Sequenom) all equally place third