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. 2017 Nov 27;20(1):32–43. doi: 10.1016/j.neo.2017.11.002

Figure 2.

Figure 2

Bo-786 culture medium inhibited osteoblast differentiation. (A) PMO cell proliferation with or without treatment with Bo-786 CM (786CM). (B) ALP gene expression (left), ALP activity (middle), and ALP staining (right) in Bo-786 CM-treated PMO cells. (C) Osteoclacin mRNA (left) and osteocalcin protein in 786-O CM-treated PMO cells (right). (D) Effect of Bo-786 CM on mineralization of PMO cells as determined by Alizarin Red S staining. (E) Proliferation of MC3T3-E1 cells with or without Bo-786 CM treatment. (F) ALP gene expression (left), ALP activity (middle), and ALP staining (right) in Bo-786 CM-treated MC3T3-E1cells. (G) Osteoclacin mRNA (left) and osteocalcin protein in 786-O CM-treated MC3T3-E1 cells (right). (H) MC3T3-E1 mineralization was determined by Alzarin Red S staining. “Con”: Control. *P < .05; **P < .01.