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. 2017 Nov 27;20(1):32–43. doi: 10.1016/j.neo.2017.11.002

Figure 3.

Figure 3

Mass spectrometry analysis of Bo-786 CM. (A) SDS-PAGE analysis of Bo-786 CM. The proteins were stained with Coomassie blue. (B) Lists of 25 secretory proteins with highest protein score from tandem mass spectrometry analysis. (C) Expression of BIGH3 in kidney cell lines as determined by real-time PCR and Western blot. (D) SDS-PAGE analysis of BIGH3-his7 protein expressed and purified from HEK293 cells (left). Western blot of purified BIGH3-his7 protein and Bo-786 CM with anti-BIGH3 antibody (right).