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. 2017 Nov 27;20(1):32–43. doi: 10.1016/j.neo.2017.11.002

Figure 5.

Figure 5

Inhibition of osteoblast differentiation by Bo-786 CM was partially mediated through BIGH3. (A) Generation of Bo-786 RCC cells with knockdown of BIGH3. Knockdown of BIGH3 was confirmed by real-time PCR, Western blot assay, and ELISA. (B) Cell proliferation and cell viability of BIGH3 knockdown cell lines. (C) Effects of CM from BIGH3 knockdown cell lines on PMO differentiation. ALP mRNA (left), ALP staining (middle), and ALP activity (right). (D) Osteocalcin mRNA and protein levels. (E) Mineralization. “NS”: 786-shNS nonsilencing control cells; “#3”: 786-shBG#3 cells; “#4”: 786-shBG#4 cells. *P < .05; **P < .01; “a”: compared to “Con”; “b”: compared to “NS”.