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. 2017 Nov 27;10:396. doi: 10.3389/fnmol.2017.00396

Figure 1.

Figure 1

Fear conditioning induced reduced astrocytic volume and Rac1 activity in basolateral amygdala (BLA). (A) Representative images of 3D reconstructed astrocytes at different time-points following fear conditioning, based on GFAP staining. The cube in white line was automatic generated by software. (B) Fear conditioning induced changes in astrocytic volume at different time-points following fear conditioning (n = 80–100 cells from 3 mice per group). (C) A representative image of Lck-mCherry and GFAP labeled cells. (D) Representative 3D reconstructed images of astrocytes that labeled with Lck-mcherry at different time-points following fear conditioning. (E) Astrocytic volume was reduced after fear conditioning (n = 60–90 cells from 3 mice per group). (F,G) GTP-bound Rac1in the BLA (F) and astrocytes (G) was enrich by binding with PAK-PBD affinity beads and analyzed on Western Blots. (H,I) Fear conditioning decreased Rac1 activity in the BLA (H) and astrocytes (I) (n = 10 per group). *p < 0.05, **p < 0.01. Scale bar, 25 μm.