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. 2017 Nov 27;8:1546. doi: 10.3389/fimmu.2017.01546

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Copyright © 2017 He, Ma and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functions of viral wsv459 and wsv322 in virus infection. (A) The expression levels of wsv459 and wsv322 in the white spot syndrome virus (WSSV)-infected shrimp. At different time postinfection, shrimp hemocytes were subjected to Northern blots. Actin was used as a control. (B) The silencing of wsv459 gene expression in shrimp. WSSV and wsv459-specific siRNA (wsv459-siRNA) or siRNA-scrambled were co-injected into shrimp. WSSV alone was used as a control. At different time postinfection, the shrimp hemocytes were collected and subjected to quantitative real-time polymerase chain reaction (PCR) to detect the wsv459 expression. (C) The knockdown of wsv322 expression in shrimp. (D) The influence of wsv459 or wsv322 silencing on the WSSV infection in shrimp. The shrimp were co-injected with wsv459-siRNA and WSSV or wsv322-siRNA and WSSV. At various time postinfection, the WSSV copies of shrimp hemolymph were determined using quantitative real-time PCR. For each treatment, three shrimp were randomly selected for real-time PCR analysis. (E) The effects of wsv459 or wsv322 silencing on the survival of WSSV-infected shrimp. Shrimp mortality was examined at different time after treatment. In all panels, the significant differences between treatments were evaluated with Student’s t-test using the results of three independent experiments for each treatment and indicated with asterisks (*p < 0.05; **p < 0.01).

Functions of viral wsv459 and wsv322 in virus infection. (A) The expression levels of wsv459 and wsv322 in the white spot syndrome virus (WSSV)-infected shrimp. At different time postinfection, shrimp hemocytes were subjected to Northern blots. Actin was used as a control. (B) The silencing of wsv459 gene expression in shrimp. WSSV and wsv459-specific siRNA (wsv459-siRNA) or siRNA-scrambled were co-injected into shrimp. WSSV alone was used as a control. At different time postinfection, the shrimp hemocytes were collected and subjected to quantitative real-time polymerase chain reaction (PCR) to detect the wsv459 expression. (C) The knockdown of wsv322 expression in shrimp. (D) The influence of wsv459 or wsv322 silencing on the WSSV infection in shrimp. The shrimp were co-injected with wsv459-siRNA and WSSV or wsv322-siRNA and WSSV. At various time postinfection, the WSSV copies of shrimp hemolymph were determined using quantitative real-time PCR. For each treatment, three shrimp were randomly selected for real-time PCR analysis. (E) The effects of wsv459 or wsv322 silencing on the survival of WSSV-infected shrimp. Shrimp mortality was examined at different time after treatment. In all panels, the significant differences between treatments were evaluated with Student’s t-test using the results of three independent experiments for each treatment and indicated with asterisks (*p < 0.05; **p < 0.01).