Fig. 2.

siRNA-TERRA enhanced telomere dysfunction. a siRNA-TERRA increased the telomerase activity in A549 cell line. b siRNA-TERRA promoted the TERT expression in A549 cell line. c The relative length of telomere was measured in A549 cells. siRNA-TERRA can promote telomere length. d siRNA-TERRA can also promote TERT in MLE-12 cell line. MLE-12 cells were transfected with siRNA-TERRA1, siRNA-TERRA2, or siRNA-negative control for 48 h, then co-treated with 60 μM of H₂O₂ for 12 h. e TERT expression was verified in the animal model. f siRNA-TERRA upregulated proliferating cell nuclear antigen expression in A549 cell line. g siRNA-TERRA enhanced cyclin D1 and cyclin E expression. A549 cells were transfected with siRNA-TERRA1, siRNA-TERRA2, or siRNA-negative control for 48 h and respectively co-treated with 120 μM of H₂O₂ for 12 h. Each bar represents mean ± SD, n = 6. ^* P < 0.05 and ^** P < 0.01 compared with the normal control group. ^## P < 0.01 compared with the H₂O₂+ siRNA-TERRA negative control group