Fig. 6.

siRNA-TERRA changed the associated gene expression. a p53, bax, and Bcl-2 were analyzed by qRT-PCR in A549. siRNA-TERRA-treated cells exhibited reduced activities of p53 and bax but increased Bcl-2 at mRNA level. b p53, bax, and Bcl-2 were analyzed by Western blot in A549. siRNA-TERRA-treated cells possessed decreased activities of p53 and bax but increased Bcl-2 at protein levels. c The activities of puma and (d) cytc were reduced in siRNA-TERRA-treated A549 cells. e Activity of cytc was also reduced in siRNA-TERRA-treated MLE-12 cells. f Caspase-3 and caspase-9 decreased markedly in siRNA-TERRA-treated A549 cells. Each bar represents mean ± SD, n = 6, ^* P < 0.05 and ^** P < 0.01 compared with the normal group; ^# P < 0.05 and ^## P < 0.01 compared with the H₂O₂+ siRNA-TERRA negative control group