Figure 3. Twenty four secondary metabolite gene clusters predicted in S. kebangsaanensis.

These gene clusters have been predicted using antiSMASH (except the phenazine biosynthetic gene cluster at scaffold 110 that was manually identified). Each colour of gene represents a different class of antibiotics and secondary metabolites, as shown in the legend. Each secondary metabolite gene cluster located in different scaffold of S. kebangsaanensis (total scaffold in the genome is 170) as shown in the numbering above the gene cluster. The genes have been ordered based on the location of predicted secondary metabolite gene cluster in the different scaffold (split loci). Phenazine biosynthetic gene cluster is located at scaffold 110 in the genome of S. kebangsaanensis. The light purple arrows represent known genes that are involved in the phenazine biosynthesis whereas the white arrows represent unknown genes that may be involved in the pathway. The numbering below the gene clusters are the location of each gene in the genome of S. kebangsaanensis (from the total of 8,001 genes based on the identified open reading frame). The forward arrows represent the forward genes whereas the reverse arrows represent the reverse genes.