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. Author manuscript; available in PMC: 2017 Dec 2.
Published in final edited form as: ACS Chem Biol. 2016 Mar 31;11(6):1587–1594. doi: 10.1021/acschembio.6b00172

Figure 3.

Figure 3

RP-HPLC traces demonstrating conjugation of [18F]-FA to the LAP peptide for (A) [18F]-FA and (B) (red trace) LAP peptide (60 μM), LplA (5 μM), [18F]-FA (~200 μCi), ATP (3 mM), and Mg(OAc)2 (5 mM) with the reaction incubated at 37 °C for 10 min and quenched via addition of EDTA to a final volume of 180 mM and analyzed by RP-HPLC using Rad-detector and (blue trace) the [19F]-LAP-FA nonradioactive standard. The HPLC eluent was 45:55:0.1 v:v:v MeCN:H2O:TFA.