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. Author manuscript; available in PMC: 2017 Dec 2.
Published in final edited form as: ACS Chem Biol. 2016 Mar 31;11(6):1587–1594. doi: 10.1021/acschembio.6b00172

Table 1.

LplA Conjugates [18F]-FA to the LAP Peptidea

peptide [peptide]/μM [LplA]/μM yield/%b
LAP 60 5 91 ± 1.5 (n = 3)
LAP 60 0 0
Sc. LAP 60 5 0
0 10 0
a

General considerations: all reactions performed in 200 μL of PBS + 3 mM ATP + 5 mM Mg(OAc)2 at 30 °C for 10 min and quenched with EDTA (180 mM final concentration) prior to analysis.

b

Radio-TLC yields measured after treatment of the reaction sample with SDS-PAGE buffer at 95 °C for 5 min. The eluent used was 7:3:0.1 EtOAc:hexanes:acetic acid.