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. Author manuscript; available in PMC: 2017 Dec 2.
Published in final edited form as: ACS Chem Biol. 2016 Mar 31;11(6):1587–1594. doi: 10.1021/acschembio.6b00172

Table 4.

Dissociation Constants, Measured Using an Octet Instrument, for 2G10-Fabs Binding to uPAR

protein KD (nM)
2G10-Fab 38 ± 2.7
2G10-Fab-LAP 31 ± 2.2
[19F]-2G10-Fab-LAPa 31 ± 2.5
a

Labeling conditions: 2G10-Fab-LAP (10 μM), LplAΔHis (50 μM), FA (750 μM), ATP (3 mM), Mg(OAc)2 (5 mM). The resulting solution was incubated at 30 °C for 15 h and then quenched with 100 μL of 360 mM EDTA.