Figure 1.

7Bio is one of the most potent chemical to inhibit Aβ oligomer-induced neuronal death in SH-SY5Y cells. (A) SH-SY5Y cells were treated with indirubin-3-oxime (I3O), indirubin-3-(2,3-dihydroxypropyl)oximether (I3DO), indirubin-3-monoxime-5-sulphonic acid (I3OS), 5-iodo-indirubin-3-monoxime (II3O), indirubin derivative E804 (E804), or 7Bio at various concentrations as indicated. After 2 h, 1.5 μM Aβ oligomers were added. The MTT assay was used to measure cell viability at 24 h after the addition of Aβ oligomers. (B) 7Bio prevents but does not rescue Aβ oligomer-induced neurotoxicity in SH-SY5Y cells. SH-SY5Y cells were exposed to 1.5 μM Aβ oligomer with 2 h pre-treatment (2 h) or co-treatment (0 h) of 10 μM 7Bio. The MTT assay was applied at 24 h after adding Aβ oligomer. Data, expressed as percentage of the control, were the mean ± SEM of three separate experiments; ^##p < 0.01 vs. the control group, ^*p < 0.05 and ^**p < 0.01 vs. Aβ oligomer group (ANOVA and Tukey's test). (C) 7Bio reduces Aβ oligomer formation. Aβ peptide was co-incubated with 7Bio at the indicated doses for 2 days. Solution was centrifuged, and the supernatants were examined via dot blotting analysis with A11, an anti-oligomer antibody, and 6E10, an anti-Aβ antibody, respectively.