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. 2017 Nov 28;8:866. doi: 10.3389/fphar.2017.00866

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Copyright © 2017 Ning, Zhan, He, Hu, Zhu, Hu, Yan, Yang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-6 decreases the expression of PXR, CAR and their target genes in primary human hepatocytes (nine individuals). (A–D) Human primary hepatocytes from nine donors were treated with IL-6 (20 ng/ml) or PBS for 24 h. Total RNA was isolated and subjected to quantitative reverse transcription-polymerase chain reaction analysis for the mRNA levels of PXR, CAR, and RXRα by TaqMan probes. (E–H) Then the mRNA level of their target genes, CYP3A4, MDR1, and CYP2B6, were detected by TaqMan probes. The signals from each target were normalized based on the signal from glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are expressed as mean ± SD. ^∗∗p < 0.01 vs. PBS.