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. 2017 Nov 28;8:866. doi: 10.3389/fphar.2017.00866

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Copyright © 2017 Ning, Zhan, He, Hu, Zhu, Hu, Yan, Yang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of DEC1 inhibits the expression and function of PXR, CAR and their targets. HepG2 cells were transfected with the FlagDEC1 construct or the corresponding vector for 48 h. The levels of PXR, CAR, RXRα, CYP3A4, MDR1, and CYP2B6 were analyzed by qRT-PCR (A,B) and Western blotting (C,D). (E) Overexpression of DEC1 inhibits the CYP3A4 activity. (F) Overexpression of DEC1 inhibits the efflux activity of MDR1. Representative histogram plots were provided with mean fluorescent intensity in the selected region of each treatment, as shown in the respective plot as the mean ± SD. To determine the transfection efficiency, cell lysates (10 μg) were analyzed for DEC1 using Western blotting. All experiments were repeated at least three times, and the data are expressed as mean ± SD. ^∗p < 0.05, ^∗∗p < 0.01 vs. vector (FlagCMV2).