Figure 4.

CD11c-dendritic cell (DC) contribute to systemic inflammation and liver damage after HS/T. (A) Flow cytometric analysis of TLR4^−/− DC generated from bone marrow precursors (day 6), purified by sorting with CD11c paramagnetic beads (Myltenyi), and adoptively transferred (i.v.) to TLR4^−/− [or wild-type (WT), control] B6 mice. The injected inoculum consisted mostly of immature DC (purity >95%). (B,C) TLR4^−/− B6 mice adoptively transferred (i.v.) with TLR4^−/− DC exhibited significantly lower concentrations of IL-6 and ALT in plasma measured 6 h after HS/T, as compared to TLR4^−/− mice injected with WT DCs. There were no significant differences in plasma concentrations of IL-6 and ALT following HS/T in WT B6 mice reconstituted (i.v.) with either WT or TLR4^−/− DCs (B) *P < 0.05, analyzed by Student’s t-test; (C) *P < 0.05, analyzed by Mann–Whitney Rank Sum Test (n = 8–12 independent experiments).