Fig. 1.

Surface expression of calreticulin and HMGB1 after co-culturing with ABAs. THP-1 cells were co-cultured overnight with various amounts of ABAs and then stained with antibodies against calreticulin, HMGB1 or an isotype control antibody. The cells were analysed by flow cytometry, and the mean channel fluorescence intensity (MFI) from the cells was measured. a Cells co-cultured with Adju-Phos and stained with APC-labelled anti-calreticulin (black circle) or isotype control (white circle). b Cells co-cultured with Adju-Phos and stained with APC-labelled anti-HMGB1 (black square) or isotype control (white square). c Cells co-cultured with Alhydrogel and stained with APC-labelled anti-calreticulin (black circle) or isotype control (white circle). d Cells co-cultured with Alhydrogel and stained with APC-labelled anti-HMGB1 (black square) or isotype control (white square)