Fig. 8.

Kinetic surface expression of calreticulin and HMGB1 after co-culturing of THP-1 cells and ABAs. THP-1 cells were co-cultured with 50 μg/ml Adju-Phos (a) or Alhydrogel (b) for various times before the cells were stained with APC labeled anti-calreticulin (black circle), anti-HMGB1 (black square) or an isotype control (black triangle). The cells were analysed by flow cytometry, and the mean channel fluorescence intensity (MFI) from the cells was measured