Figure 3.

Modulation of pro-inflammatory cytokine responses in co-stimulated term neonatal and adult monocytes. Using qRT-PCR, cytokine flow cytometry and multi-analyte immunoassay, we assessed mRNA expression (A–C), intracellular protein synthesis (D–F) as well as protein secretion (G–I) of each cytokine. LPS-activated cells served as positive control, LPS-primed monocytes exposed to Ureaplasma broth served as negative control. Values represent the means ± SD (^#p < 0.05, ^##p < 0.01, vs. broth control; °p < 0.05, ^°°p < 0.01, vs. LPS-activated monocytes).