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. 2017 Nov 28;8:2321. doi: 10.3389/fmicb.2017.02321

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Copyright © 2017 Flynn, Koval, Greenwald, Owens, Kemner and Antonopoulos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Conceptual diagram of our experimental workflow. (1) Collection of inocula for substrate arrays. (2) Dilute suspension of soil/water microbes prepared within 72 h. (3) Substrate arrays where each well is amended with one of 31 individual carbon sources (in triplicate), inoculated with suspension (1:100 m/v dilution), and incubated for 72 h. (4) Changes in composition of microbial communities in the substrate array is assayed by sequencing 16S rRNA gene amplicons on the Illumina MiSeq platform.