Figure 3.

Novel Object Recognition test (NOR). Wistar rats were given access to ethanol (20% in drinking water) or tap water for 2-h sessions during 3 days, and for an additional 4-h session on the 4th day, every week during adolescence, from pnd 28–52. At adulthood (pnd 63), animals were submitted to the NOR test. The discrimination index (DI) was calculated as the difference between the time spent exploring the novel object (N) and the familiar one (F1 or F2) in relation to the total time spent exploring the objects ((N − F)/(N + F)). Data are presented as mean ± SEM. Analysis of variance (ANOVA), ^bp < 0.05, general effect of treatment. n = 13–15 animals per experimental group, coming from eight litters.