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. 2018 Jan;22(1):42–49. doi: 10.22034/ibj.22.1.42

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Copyright: © Iranian Biomedical Journal

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — PCR pattern of G and P type human rotavirus. PCR amplicon of rotavirus typing (A) Representative agarose gel electrophoresis of PCR products of semi-multiplex P typing. Lanes 1, 10, and 20, 100 bp ladder; lane 18, RV4 (P8) representing positive control; lanes 3, 8, 11, 12, 17, and 19, P[4] genotype; lanes 5, 7, 14, 15, and 16, P[8] genotype; lane 2, negative control. Lanes 4, 9, and 13, negative results. (B) Representative agarose gel electrophoresis of PCR products of semi-multiplex G typing. Lanes 1, 10, and 20, 100 bp ladder; lane 2, RV4 (G1) representing positive control; lanes 4, 5, and 6, G1 genotype (749 bp); lanes 3, 8, 9, 11, 12, and 19, G2 genotype (652 bp); lanes 7, 14, 15, and 18, G9 (305 bp); lanes 17, 16, and 13, negative results.