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. 2017 Nov 28;8:2052. doi: 10.3389/fpls.2017.02052

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Copyright © 2017 Cao, Wang, Nawaz, Niu, Sun, Xie, Kong, Huang, Cheng and Bie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression patterns of CmNAC1 in pumpkin seedlings under different stress treatments or in different organs. (A) Expression patterns of CmNAC1 in various organs (root, RT; young leaves, YL; mature leaves, ML; cotyledon, CN; hypocotyl, HL; stem, ST; fruit, FT). Expression patterns of CmNAC1 in leaves after H₂O₂ (B), NaCl (C), GA (D), cold (E), ABA (F) and dehydration (G) treatments by qRT-PCR analysis. Zero represents leaf sample without any treatment; 0.5, 1, 4, 8, and 12 represent samples after 0.5, 1, 4, 8, and 12 h treatment, respectively. The 2^-ΔΔCT method was used in qRT-PCR analysis. Transcript levels were normalized to CmEF1α and CmCAC. Values are means ± SD of three replicates. Three independent experiments were performed. Asterisks indicate a significant difference (^∗P < 0.05; ^∗∗P < 0.01) compared with the corresponding controls.