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. 2017 Nov 28;8:2052. doi: 10.3389/fpls.2017.02052

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Copyright © 2017 Cao, Wang, Nawaz, Niu, Sun, Xie, Kong, Huang, Cheng and Bie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transactivation assay of CmNAC1 in yeast cell Y2HGold. (A) Full-length protein (CmNAC1-A), N-terminal fragment (CmNAC1-N) and C-terminal fragment (CmNAC1-C) were fused with GAL4 DNA binding domain and expressed in yeast strain Y2HGold. The pGBDKT7 vector was used as a negative control. (B) Transformed yeasts were dripped on the SD/-Trp, SD/-Trp-His-Ade, and SD/-Trp-His-x-gal after being cultured for 3 days in the growth chamber.