Table 3.

Differentially expressed virulence-related genes in C. sakazakii ATCC 29544 grown in TSB with TQ at different concentrations.

Target gene	Sequence of primers(5′-3′)c	Relative gene expression
		400 μmol/L	600 μmol/L
ESA_04030	F, CCAGGGCTACACACGTGCTA	1	1
	R, TCTCGCGAGGTCGCTTCT
bcsA	F, CACGATGGTGGCGTTGTTC	−1.74 ± 0.3a	−4.62 ± 1.49a
	R, CCTTTGGCGGTGACGTTAA
bcsG	F, ACGACTGGCTGAACAGCTTTTAC	−1.32 ± 0.37	−4.74 ± 0.23b
	R, GCCGGGAAGGTTGTCTGA
flhD	F, CGATGTTTCGCCTGGGAAT	−1.10 ± 0.06a	−2.66 ± 0.16b
	R, AGAGTCAGGTCGCCCAGTGT
fliD	F, AAAACCGCAACATGGAATTCA	−1.78 ± 0.13b	−7.29 ± 0.57b
	R, CCGCAAACGCGGTATTG
flgJ	F, GACGGCGGGCAAAGG	−1.12 ± 0.62	−3.17 ± 0.52b
	R, GCCGCCCATCTGTTTGAC
motA	F, GGTGTGGGTGCGTTTATCGT	−1.06 ± 0.01a	−2.83 ± 0.38b
	R, GCCTTCAGCGTGCCTTTG
motB	F, ACGGCTCGTGGAAAATCG	−1.09 ± 0.02a	−2.54 ± 0.21b
	R, CCAGGAAGAAGGCCATCATG
luxR	F, TGTGCGTTCGCCATCCT	−1.23 ± 0.10a	−4.91 ± 1.81a
	R, TGGTGTGCAGCGTCAGTTTT
lpxB	F,GCACGACACTTTCCGTAAACTG	−1.05 ± 0.02b	−1.69 ± 0.12b
	R,CGCCTGTTCATCGGCATT
ompA	F,GGCCGCATGCCGTATAAA	−2.15 ± 0.01b	−2.70 ± 0.37b
	R,GCTGTACGCCCTGAGCTTTG
ompX	F,GTCTTTCAGCACTGGCTTGTGT	−1.51 ± 0.16b	−4.13 ± 0.68b
	R,GGTGCCAGCAACAGCAGAA
sod	F,CGAATCTGCCGGTTGAAGA	−1.36 ± 0.14a	−1.99 ± 0.08b
	R,CTTGTCCGCCGGAACCT
uvrY	F,GCGAGGACGCCATCAAAT	−1.57 ± 0.25a	−2.12 ± 0.48a
	R,ATCCATCAGCACCACATCCA
wzx	F,TGCTTGGGCAGGTACAAAGTG	−1.39 ± 0.08b	−4.28 ± 0.90b
	R,CCCTACGGGTGCAGTCACA
galE	F,CTTGAGTATTACGACAACAACG	−3.39 ± 0.29b	−3.94 ± 0.35b
	R,GAAACTTTCGACATAAGGGAT
kpsT	F,ATTGGCGGGACGGATAA	−2.50 ± 0.19b	−4.85 ± 0.33b
	R,TCGTCCACCAGGTAGTAGTCA

^aP ≤ 0.05,

^bP ≤ 0.01,

^cF, forward; R, reverse.