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. Author manuscript; available in PMC: 2017 Dec 3.
Published in final edited form as: Biochem Pharmacol. 2017 Aug 16;145:178–191. doi: 10.1016/j.bcp.2017.08.012

Fig. 6.

Fig. 6

Hsa-miR-132-5p regulates endogenous and lansoprazole-induced exogenous CYP1A2 expression in Huh-7 and HepG2 cells. (A) Relative CYP1A2 mRNA expression in Huh-7 cell when induced by lansoprazole (10 and 50 μM, dissolved in DMSO) at different time points (0, 4, 12, and 24 h), and GAPDH mRNA expression was used as an internal control. (B) Representative Western blot analysis shows the CYP1A2 expression in Huh-7 cells induced by lansoprazole (10 and 50 μM) at different time points (0, 4, 12, and 24 h), and GAPDH was used as loading control. (C) The bar graphs represent the densitometry analysis of CYP1A2 protein expression from five independent experiments (mean ± SEM) and the intensities of bands were normalized to the amount of GAPDH (*, P < 0.05). (D, E, F) Induction of CYP1A2 by lansoprazole was modulated by hsa-miR-132-5p in both mRNA and protein levels in Huh-7 cells, and GAPDH was used as an internal control. (G, H, I) Lansoprazole-induced exogenous CYP1A2 expression in HepG2 cells could be regulated by hsa-miR-132-5p, and GAPDH was used as an internal control. Error bars represent ± SEM (n = 5); *, P < 0.05.