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. Author manuscript; available in PMC: 2017 Dec 3.
Published in final edited form as: Nat Immunol. 2017 Jul 31;18(9):995–1003. doi: 10.1038/ni.3809

Figure 6.

Figure 6

Human NK cells with a SMAD4 mutation are hyper-responsive to TGF-β. (a) Immunoblot analysis of total SMAD4 and actin (loading control) in human NK cells from a SMAD4-deficient patient (PT00) and a healthy control donor (Ctrl1). (b) Flow cytometry (left) and quantification (right) of CD103 and CD9 in NK cells from donors as in a and cultured with TGF-β1 plus IL-2 or with IL-2 alone. (c) Expression of ZNF683 (encoding Hobit), ITGAE (encoding CD103), CD9 (encoding CD9) and IFNG (encoding IFN-γ) (left margin) in NK cells from donors as in a and cultured overnight with TGF-β1 and IL-2 or with IL-2 alone. (d) Secretion of IFN-γ by NK cells from donors as in a (two healthy donors: Ctrl1 and Ctrl2) cultured overnight alone (Medium) or together with K562 target cells. ***P < 0.001 (unpaired Student’s t-test). Data are representative of two (a), QQ (c) or at least three (b,d) independent experiments (mean + s.d. in d).