Fig. 1. Genetic labeling of Sox12+ vs Sox12− HCC cells with different fluorescence and luciferase.

(A) Two human HCC cell lines SNU-182 and HepG2 were co-transduced with 2 lentiviruses. The first lentivirus carries a RFP reporter under the control of a Sox12 promoter (pSox12-RFP). The second lentivirus carries a luciferase (LUC) reporter and a GFP reporter under a CMV promoter (pCMV-LUC-GFP). The viral structure is shown. (B) Representative flow cytometry for GFP and RFP in the HCC cells infected with both lentiviruses. (C) RT-qPCR for the purified GFP+RFP− and GFP+RFP+ cells, compared to total GFP+ cells. (D) The purified GFP+ (RFP+ or FRP−) HCC cells in culture. DAPI: nuclei staining. Scale bars are 50 μm.