Fig. 1.

Retinal SIRT3 was mainly expressed in mitochondria, and the level was decreased with age. a, b In situ hybridization of Sirt3 in the retina of 10-week-old C57BL6/J mice. Scale bars: a 20 μm b 200 μm. GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, OS outer segment, RPE retinal pigment epithelium, AS antisense, S sense. c Representative Immunoblot of SIRT3 for subcellular fraction of the retina of 10-week-old C57BL6/J mice. T total retina, C cytoplasmic, M mitochondrial, N nuclear, CM cytoplasmic and mitochondrial fractions. d–g Immunoblot of young (10 weeks old) and old (60 weeks old) C57BL6/J mice retina against nicotinamide phosphoribosyltransferase (NAMPT) e, SIRT1 f, and SIRT3 g (n = 5 for each group). *p < 0.05 by two-tailed unpaired t-test. Values are mean ± SD