Fig. 2.

SIRT3 KO retinas showed increased acetylated protein and damaged mitochondria. a Representative images of Immunoblot of mitochondrial fraction isolated from 10-week-old WT and SIRT3 KO retinas against Acetylated-Lysine (Ac-K). b, c Representative HE staining images of b young (10 weeks old) and c old (60 weeks old) WT and SIRT3 KO mice retinas. Scale bars: 20 μm. GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, OS outer segment, RPE retinal pigment epithelium. d, e Electromicroscopy analysis of the morphology of mitochondria in the photoreceptor inner segments of 10-week-old WT and SIRT3 KO retinas. Mitochondria of WT retinas exhibited regularly spaced and densely packed cristae, whereas mitochondria of SIRT3 KO retinas exhibited sparse with irregularly arranged cristae. Scale bars: d 1 μm e 200 nm