Fig. 5.

SIRT3 KO retinas showed increased reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress after the light exposure (LE). a, b Comparison of in vivo DHE staining for WT and SIRT3 KO outer nuclear layers (ONL) without and with LE (1 h after LE; WT n = 4, KO n = 4, WT LE n = 4, KO LE n = 5). Scale bars: 20 μm. *p < 0.05, by one-way ANOVA with post hoc Tukey’s HSD test. c Representative images of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stain for SIRT3 KO retinas 36 h after the light exposure with control vehicle or N-Acetyl-l-cysteine (NAC) treatment. Blue: DAPI, Red: TUNEL. Scale bars: 20 μm. ONL outer nuclear layer. d Comparison of TUNEL-positive cell counts per section (n = 4 for control group and n = 6 for NAC treatment group). ***p < 0.001 by two-tailed unpaired t-test. e, f Expression of ER stress markers in WT and SIRT3 KO retinas without and with LE. Relative mRNA expression of e Bip and f Chop in the retina (1 h after LE; WT: n = 4, KO: n = 4, WT LE: n = 4, KO LE: n = 5). **p < 0.01 and ***p < 0.001 by one-way ANOVA with post hoc Tukey’s HSD test. Values are mean ± SD