Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 29;7:294. doi: 10.3389/fonc.2017.00294

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Mazzoni, Rotondo, Marracino, Selvatici, Bononi, Torreggiani, Touzé, Martini and Tognon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Merkel cell polyomavirus (MCPyV) DNA sequences in blood donor serum samples. (A) Schematic representation of the primers and probe employed in polymerase chain reaction (PCR), quantitative PCR, and droplet digital PCR (ddPCR) methods. (B) Agarose gel electrophoresis of PCR-amplified MCPyV large T antigen (LT) sequences, stained by ethidium bromide. M, molecular weight markers (100 bp). Lane R+, positive control represented by the recombinant plasmid pUC57MC1 carrying Merkel cell carcinomas 350 DNA sequences (17, 20). Lanes 1–5, DNA samples extracted from blood donor serum samples (number, n) (n177, n179, n54, n219, and n284). Lane R−, negative control of PCR reactions without DNA template. The arrow indicates the product size of 138 bp. (C) MCPyV copy number. MCPyV DNA load in blood donor serum samples is indicated as copy number per microliter of serum (left). Horizontal and vertical bars indicate the mean value and the SD, respectively.