Table 1.

Primer sets employed by polymerase chain reaction (PCR) techniques to detect and quantify Merkel cell polyomavirus DNA sequences in serum samples from blood donors.

Primer sets	Nucleotide sequence position	Sequence 5'–3'	PCR amplicon (bp)
PCR and ddPCR
MCPyLT1709.F	1,709–1,732	CAGGCATGCCTGTGAATTAGGATG	138
MCPyLT1846.R	1,846–1,827	TCAGGCATCTTATTCACTCC
Q-PCR
LT.1F	1,034–1,053	CCACAGCCAGAGCTCTTCCT	146
LT.1R	1,179–1,157	TGGTGGTCTCCTCTCTGCTACTG
LT. probe	1,065–1,088	FAM-TCCTTCTCAGCGTCCCAGGCTTCA-TAMRA