Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Characteristics1 | Source |
|---|---|---|
| Streptomyces chartreusis | ||
| NRRL 3882 | A23187 production, wild-type | NRRL |
| GLX26 (ΔcalR3) | calR3-deletion mutant | This work |
| GLX29 (ΔcalR3+calR3) | ΔcalR3 complementation strain | This work |
| Escherichia coli strains | ||
| DH10B | F- recA lacZ ΔM15 | Invitrogen |
| ET12567(pUZ8002) | Cml, Kan, dam dcm hsdS Tra+ Cml | Kieser et al., 2000 |
| BW25113/pIJ790 | RepA101(ts), araBp-gam-bet-exo, AraC, Cml | Kieser et al., 2000 |
| BL21(DE3)/plysE | F- dcm ompT hsdS (rB- mB-) gal | Stratagene |
| (DE3) [pLysE Cml] | ||
| Plasmids | ||
| pBluescript II SK(+) | bla, lacZ, orif1 | Stratagene |
| pJTU2170 | aac(3)IV, lacz, reppuc, attΦC31, oriT | Huang et al., 2011 |
| pET28a(+) | Plasmid for gene expression | Novagen |
| p16F9 | Cml | Wu et al., 2011 |
| pJTU3791 | p16F9 derived plasmid carrying a apramycin resistance gene and a defective calR3 | This work |
| pJTU3795 | pJTU2170 derived plasmid carrying calR3 for expression in Streptomyces | This work |
| pET28a-calR3 | pET28a(+) derived plasmid | This work |
| for calR3 expression | ||
1Cml, chloramphenicol resistance; Kan, kanamycin resistance; aac(3)IV, apramycin resistance.