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Alternate-day fasting alleviated the increase of AQP4-M1/M23 ratio in the cerebral cortex of APP/PS1 mice. The relative expression of AQP4 mRNA (β-actin as a reference standard) (A) and proteins (B,C) were analyzed by qRT-PCR and Western blot, respectively (n = 9 or 10; mean ± SD; one-way ANOVA followed by LSD multiple comparison tests; ∗p < 0.05, ∗∗p < 0.01 vs. WT group, #p < 0.05, ##p < 0.01 vs. AD group).
