FIGURE 5.

Alternate-day fasting alleviated the decrease of miR-130a expression and the increase of HDAC3 expression in the cerebral cortex of APP/PS1 mice. The relative expression of miR-130a (A; U6 as a reference standard), HDAC3 mRNA (B; β-actin as a reference standard), and proteins (C,D) were analyzed by qRT-PCR and Western blot, respectively (n = 9 or 10; mean ± SD; one-way ANOVA followed by LSD multiple comparison tests; ^∗p < 0.05, ^∗∗p < 0.01 vs. WT group, ^#p < 0.05, ^##p < 0.01 vs. AD group).