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. 2017 Nov 29;10:395. doi: 10.3389/fnmol.2017.00395

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Copyright © 2017 Zhang, Zhan, Li, Xing, Jiang, Chen, Shi and An.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing HDAC3 caused the up-regulation of miR-130a expression and reduction of AQP4-M1/M23 ratio in U251 cells. siRNA duplex or RGFP966 was used to interfere with endogenous HDAC3 expression (untreated cells and non-specific siRNA as controls). The relative expression of miR-130a (A; U6 as a reference standard), AQP4 mRNA (A; β-actin as a reference standard), and proteins (B,C) expression were, respectively, analyzed by qRT-PCR and Western blot in HDAC3-silenced cells (n = 6; mean ± SD; one-way ANOVA followed by LSD multiple comparison tests; ^∗∗p < 0.01 vs. control group, ^##p < 0.01 vs. scrambled siRNA group).