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. 2017 Nov 29;8:966. doi: 10.3389/fphys.2017.00966

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Copyright © 2017 Ning, Li, Cheng, Yu, Rao, Ruan, Yuan, Zhang, Zhuo, Du and Xiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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High expression of TRPV4 in testicular IRI correlates with downregulation of miR-29a. (A) Immunohistochemistry staining of TRPV4 in the testis exposed to 1 h ischemia followed by reperfusion of different durations. n = 5 per group; (B–E) QRT-PCR and western blot analysis of miR-29a and TRPV4 expression at different reperfusion times after 1 h ischemia in animal samples. ^*p < 0.05 vs. sham, n = 5 per group; (F) A two-tailed Pearson's correlation analysis reveals that the mRNA expression of miR-29a is inversely correlated with the expression of TRPV4 (p < 0.05).